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| Seasonal production of moulting hormone in the barnacle Semibalanus balanoides [An article from: Journal of Experimental Marine Biology and Ecology] | ![Seasonal production of moulting hormone in the barnacle Semibalanus balanoides [An article from: Journal of Experimental Marine Biology and Ecology]](http://ecx.images-amazon.com/images/I/51NC8MRHJ0L._SL160_.jpg)
 enlarge | Authors: E.d. Morgan, C.r. Bielby, I.d. Wilson
Publisher: Elsevier
Category: Book
Buy New: $8.95
Format: Html
Media: Digital
ASIN: B000RR89XQ
Availability: Available for download now
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Product Description
This digital document is a journal article from Journal of Experimental Marine Biology and Ecology, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
An analytical procedure for the quantification of ecdysteroids (crustacean moulting hormone) in barnacles was devised so that minimum sample size could be used. A combination of solvent partitions, Sephadex chromatography, silylation and gas chromatography with electron capture detection was devised, enabling ecdysteroids to be determined down to 20 pg. This was used to determine the amount of moulting hormone in a population of barnacles over a 30 month period. Levels varied from barely detectable in winter months to a maximum value of 1.5 @mg kg^-^1 of wet weight of barnacles in September. Polar conjugates of 20-hydroxyecdysone were detected only during the winter months. The number of barnacles moulting at any time corresponded roughly to the titre of hormone present at that time.
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